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Project
BOBS
Part:BBa_J45900:Design
Designed by: Stephen Payne Group: iGEM06_MIT (2008-02-04)
Banana odor biosynthetic system
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal BglII site found at 3267
Illegal BamHI site found at 4594 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
Illegal AgeI site found at 1114 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3694
Illegal SapI.rc site found at 4702
Design Notes
The BBa_J45009 contains BioBrick restriction sites EcoRI and SpeI. Thus, BBa_J45009 can only be used as a suffix part in BioBrick assembly.
Source
BBa_J45200 and BBa_J45400.
References
See Part:BBa_J45014:Design#References, Part:BBa_J45008:Design#References and Part:BBa_J45009:Design#References.