Project
BOBS

Part:BBa_J45900:Design

Designed by: Stephen Payne   Group: iGEM06_MIT   (2008-02-04)

Banana odor biosynthetic system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal BglII site found at 3267
    Illegal BamHI site found at 4594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3694
    Illegal SapI.rc site found at 4702


Design Notes

The BBa_J45009 contains BioBrick restriction sites EcoRI and SpeI. Thus, BBa_J45009 can only be used as a suffix part in BioBrick assembly.

Source

BBa_J45200 and BBa_J45400.

References

See Part:BBa_J45014:Design#References, Part:BBa_J45008:Design#References and Part:BBa_J45009:Design#References.